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2024-02-12 18:31:00 | onclick: | Scientists develop non-coding evolution technology of endogenous genes

Recently, the research and innovation team of crop pathogenic biological functional genomes of the Institute of Plant Protection of the Chinese Academy of Agricultural Sciences developed a nuclease LbCas12a-mediated non-coding region-directed evolution technology.The findings are published in Plant Communications.
A large number of natural or random mutagenesis non-coding region variations contribute to crop domestication and breeding.At present, the non-coding region of endogenous genes in crops is mainly edited and modified by nuclease SpCas9.Compared to SpCas9, LbCas12a-mediated endogenous gene non-coding region techniques are more likely to recognize thymine-rich gene sequences and induce larger deletions in plant cells, better suited for non-coding region editing and directed evolution.
By using LbCas12a-mediated gene editing technology and crRNA fusion library, large-scale saturation coverage of the non-coding region of high endogenous gene SD1 in rice plants was created.This technology provides technical support for the research of crop quantitative character variation creation and molecular breeding.
The research was funded by the National Natural Science Foundation, Science and Technology Innovation 2030 - Major Projects, Hainan Yazhou Bay Seed Laboratory, and the Chinese Academy of Agricultural Sciences' Youth Innovation Special Project.(Correspondent: Kwok Kin-ying)

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